| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome
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| Autores: |
Clerissi, Camille
De Lorgeril, Julien
Petton, Bruno
Lucasson, Aude
Escoubas, Jean Michel
Gueguen, Yannick
Dégremont, Lionel
Mitta, Guillaume
Toulza, Eve
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| Data: |
2020-02
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| Ano: |
2020
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| Palavras-chave: |
Holobiont
Microbiome
Metabarcoding
Fitness
Bacteria
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| Resumo: |
Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 μVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from each family were either sampled for individual microbiota analyses using 16S rRNA gene-metabarcoding or transferred into facilities to record their survival using controlled condition. As expected, all oysters from susceptible families died, and all oysters from the resistant family survived. Quantification of OsHV-1 and bacteria showed that 5 days of transplantation were long enough to contaminate oysters by POMS, but not for entering the pathogenesis process. Thus, it was possible to compare microbiota characteristics between resistant and susceptible oysters families at the early steps of infection. Strikingly, we found that microbiota evenness and abundances of Cyanobacteria (Subsection III, family I), Mycoplasmataceae, Rhodobacteraceae, and Rhodospirillaceae were significantly different between resistant and susceptible oyster families. We concluded that these microbiota characteristics might predict oyster mortalities.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00621/73332/72500.pdf
https://archimer.ifremer.fr/doc/00621/73332/72504.pdf
https://archimer.ifremer.fr/doc/00621/73332/72507.tiff
https://archimer.ifremer.fr/doc/00621/73332/72509.tiff
https://archimer.ifremer.fr/doc/00621/73332/72510.tiff
https://archimer.ifremer.fr/doc/00621/73332/72511.xlsx
https://archimer.ifremer.fr/doc/00621/73332/72513.xlsx
https://archimer.ifremer.fr/doc/00621/73332/72517.xlsx
https://archimer.ifremer.fr/doc/00621/73332/72518.xlsx
https://archimer.ifremer.fr/doc/00621/73332/72519.xlsx
DOI:10.3389/fmicb.2020.00311
https://archimer.ifremer.fr/doc/00621/73332/
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| Editor: |
Frontiers Media SA
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI
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| Formato: |
application/pdf
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| Fonte: |
Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2020-02 , Vol. 11 , N. 311 , P. 11p.
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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